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Composite

Part:BBa_K737041:Design

Designed by: Wenjie Wu and Peiran Zhang   Group: iGEM12_OUC-China   (2012-09-19)

galK::GFP generator ligated to LacI generator and constitutive sRNA device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1545
    Illegal NheI site found at 1568
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2357


Design Notes

No


Source

No

References

[1] Boris Görke and Jörg Vogel, Noncoding RNA control of the making and breaking of sugars, GENES & DEVELOPMENT 22:2914–2925 ,2008